
蛋白G Agarose (High Affinity) (ab193258)
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产品名称 | 蛋白G Agarose (High Affinity) |
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品牌 | 艾博抗Abcam |
货号 | ab193258 |
样品类型 | Cell culture supernatant, Serum, Cell culture media, Ascites Fluid |
产品概述 | Very high binding capacity (>30 mg IgG/mL). Minimal leaching of ligand. Suitable for column or batch purification of IgG, immunoprecipitation & ChIP (ab193258).Contents: Supplied as 50% slurry in 20% Ethanol. Features:High binding capacity: Binding of IgG ≥ 30 mg human or rabbit IgG/mL Protein G Agarose.Minimal leaching of the ligandFlow Rate Tested*: 2.89 mL/min.*Test condition: = Calculations based on the time required to pass 18 mL of water through 2 mL settled beads (column diameter 1.5 cm).Maximum Flow Rate**: 1800 cm/hr; minimum leaching of recombinant Protein G.**NOTE: the highest flow that beads withstand for 1 minute, without collapsing and the pressure reaching 1 MPa.Usage:Reusable for up to 10 times without significant loss of binding capacity.These beads are for use in column purification. If used in batch purification, we recommend not exceeding 150 x g when centrifuging.Store beads at 4°C.The beads may be damaged above 40°C.DO NOT FREEZE.Wash beads 3 times with 3x bead volume of desired buffer before use. Applications:- Purification of monoclonal and polyclonal antibodies from culture media, serum, ascites fluid or hybridoma supernatants.- Isolation of antibody/antigen complexes in immunoprecipitation experiments, since only the Fc region is involved in antibody binding and the Fab region is available for binding antigen. |
经测试应用 | 适用于: IP, Purification |
研究领域 | Kits/ Lysates/ Other Kits Molecular Biology Kits Beads Protein G |
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