MeDIP Ultra试剂盒(ab185904)
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| 产品名称 | MeDIP Ultra试剂盒 |
|---|---|
| 品牌 | 艾博抗Abcam |
| 货号 | ab185904 |
| 样品类型 | DNA |
| 检测时间 | 3h 00m |
| 产品概述 | The MeDIP Ultra Kit is a complete set of optimized reagents to enrich and capture methylated DNA fragments in a convenient microplate-based format. The method, methylated DNA immunoprecipitation (MeDIP), uses a monoclonal antibody specific to 5-methylcytosine to immunoprecipitate methylated genomic DNA. The enriched methylated fractions can then be used for gene-specific DNA methylation analysis on a genome wide scale. The highly sensitive and specific format of the kit can use DNA isolated from various species. The methylated DNA that is enriched with this kit can be used for various downstream applications including qualitative and quantitative PCR (MeDIP-PCR), microarray (MeDIP-chip) and especially sequencing (MeDIP-seq). Starting Materials, Input Amount, & Expected YieldThe starting material should be good quality purified DNA. The amount of DNA for each reaction can be 50 ng (approximately 10,000 cells) to 500 ng. For an optimal reaction, the input DNA amount should be 100-200 ng per well. The yielded methylated DNA is about 4 ng for 100 ng input DNA (4%), which is consistent with the expected percentage (4-5%) at which the highest sensitivity and specificity for enriched methylated DNA has been demonstrated by bisulfite sequencing. |
| 研究领域 | Kits/ Lysates/ Other Kits Epigenetic kits DNA modification and methylation |
|---|
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