
Myeloperoxidase (MPO)抑制剂Screening Assay试剂盒(ab133080)
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产品名称 | Myeloperoxidase (MPO)抑制剂Screening Assay试剂盒 |
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品牌 | 艾博抗Abcam |
货号 | ab133080 |
精确度 | 批次内 样品 n Mean SD CV% Chlorination 16 3.7% Peroxidation 16 2.8% 批次间 样品 n Mean SD CV% Chlorination 16 3.6% Peroxidation 16 3.1% |
种属反应性 | 与反应: Human 预测可用于: Mammals |
产品概述 | Abcam’s Myeloperoxidase (MPO) Inhibitor Screening Assay (ab133080) provides convenient fluorescence-based methods for screening inhibitors to both the chlorination and peroxidation activities of MPO. The chlorination assay utilizes the non-fluorescent 2-[6-(4-aminophenoxy)-3-oxo-3H-xanthen-9-yl]-benzoic acid (APF), which is selectively cleaved by hypochlorite (-OCl) to yield the highly fluorescent compound fluorescein. Fluorescein fluorescence is analyzed with an excitation wavelength of 480-490 nm and an emission wavelength of 515-520 nm. The peroxidation assay utilizes the peroxidase component of MPO, where a single two electron oxidation of native enzyme (MPO) to compound I (MPO-I) is followed by two successive one electron reductions back to native enzyme by compound II (MPO-II). The reaction between hydrogen peroxide and ADHP (10-acetyl-3,7-dihydroxyphenoxazine) produces the highly fluorescent compound resorufin. Resorufin fluorescence is analyzed with an excitation wavelength of 530-540 nm and an emission wavelength of 585-595 nm. |
研究领域 | Immunology Innate Immunity Macrophage / Inflamm. Cardiovascular Blood Other Cardiovascular Vasculature Vasculature Markers Arterial Cardiovascular Vasculature Vasculature Markers Pulmonary Immunology Innate Immunity Myeloid Cells Cancer Cancer Metabolism Cellular metabolic process Kits/ Lysates/ Other Kits Cell Damage Kits Oxidative stress Kits/ Lysates/ Other Kits ELISA Kits ELISA Kits Cancer proteins ELISA kits Kits/ Lysates/ Other Kits ELISA Kits ELISA Kits Cardiovascular ELISA kits Metabolism Pathways and Processes Redox metabolism Antioxidants |
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