大鼠IgG ELISA试剂盒，Fluorescent (ab229388)
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| 产品名称 | 大鼠IgG ELISA试剂盒，Fluorescent |
|---|---|
| 品牌 | 艾博抗Abcam |
| 货号 | ab229388 |
| 精确度 | 批次内 样品 n Mean SD CV% Rat serum 3 2.66% 批次间 样品 n Mean SD CV% Rat serum 3 3.61% |
| 样品类型 | Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma |
| 灵敏度 | 35 pg/ml |
| 范围 | 0.04 ng/ml - 160 ng/ml |
| 回收率 | 特定样本回收率 样品类型 平均% 范围 Antibody Diluent 4B 103.7 99.4% - 110.97% 10F DMEM Medium 79.1 67.5% - 98% OF DMEM Medium 76.5 62.8% - 94% |
| 检测时间 | 1h 30m |
| 种属反应性 | 与反应: Rat不与反应: Goat, Cow, Pig |
| 产品概述 | Immunoglobulin G (IgG) in vitro CatchPoint SimpleStep ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Immunoglobulin G (IgG) protein in rat serum, plasma, and cell culture supernatant.This CatchPoint SimpleStep ELISA kit has been optimized for Molecular Devices Microplate Readers. Click here for a list of recommended Microplate Readers.If using a Molecular Devices’ plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and analysis settings at www.softmaxpro.org.The CatchPoint SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission. |
| 研究领域 | Immunology Immunoglobulins Heavy Chain IgG Secondary antibodies anti-Rat IgG Fluorophore FITC |
|---|
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